23EG

Artificial Copper-binding dimeric protein 1 (Cu(I)DP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2025-11-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	115.629, 52.116, 79.322
Unit cell angles	90.00, 114.14, 90.00

Refinement procedure

Resolution	29.150 - 2.670
R-factor	0.2661
Rwork	0.263
R-free	0.32210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.465
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.150	2.770
High resolution limit [Å]	2.670	2.670
Number of reflections	12305	1865
<I/σ(I)>	9.48
Completeness [%]	98.6
Redundancy	6.58
CC(1/2)	0.991	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris-HCl (pH 8.5) buffer with 0.2 M NaCl and 17 % (w/v) PEG 3350