23EF

Artificial Copper-binding dimeric protein 2 (Cu(II)DP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2025-09-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	46.450, 51.024, 52.028
Unit cell angles	101.54, 109.77, 98.12

Refinement procedure

Resolution	36.400 - 1.890
R-factor	0.1868
Rwork	0.185
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.400	1.961
High resolution limit [Å]	1.890	1.890
Rpim	0.028
Number of reflections	33086	1640
<I/σ(I)>	17.2
Completeness [%]	96.8
Redundancy	3.5
CC(1/2)	0.996	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES (pH 7.5) buffer with 0.2 M ammonium acetate and 22% (w/v) PEG 3350