232L
T4 LYSOZYME MUTANT M120K
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-01 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.930, 60.930, 96.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.730 |
| Rwork | 0.173 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.390 |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.700 | 1.800 |
| Rmerge | 0.046 | 0.172 |
| Number of reflections | 14179 | |
| <I/σ(I)> | 12.9 | 3.1 |
| Completeness [%] | 67.0 | 59.4 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 4 * | protein solution is mixed in a 1:1 ratio with precipitant solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 2 | 1 | drop | 0.55 (M) | ||
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | oxidized beta-mercaptoethanol | 50 (mM) | precipitant |
| 5 | 1 | reservoir | reduced beta-mercaptoethanol | 50 (mM) | precipitant |
| 6 | 1 | reservoir | sodium/potassium phosphate | 1.8-2.0 (M) |
