232D
THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 32.700, 32.700, 78.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.139 * |
| R-free | 0.21600 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 * | |
| High resolution limit [Å] | 1.300 | 1.300 * |
| Rmerge | 0.063 | 0.340 * |
| Number of reflections | 6494 | |
| Completeness [%] | 98.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 287.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 5 | 1 | 1 | NH4OH | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 3 (mM) | 0.005ml in 30 mM sodium cacodylate |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | 35 (%(w/v)) | 0.005ml | |
| 4 | 1 | drop | hexammine-pentanediol | 10 (mM) | 0.003ml |
| 5 | 1 | drop | MPD | 50 (%(v/v)) | 0.002ml |
| 6 | 1 | reservoir | MPD | 50 (%(v/v)) |
