231D
STRUCTURE OF A DNA-PORPHYRIN COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 296 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 39.490, 39.490, 56.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.160 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 1334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 3 | WATER | ||
| 9 | 1 | 3 | K2PTCL4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | d(CGAT1CG) | 0.5 (mM) | |
| 2 | 1 | drop | CuTMPyP4 | 0.5 (mM) | |
| 3 | 1 | drop | MPD | 0.5 (%) | |
| 4 | 1 | drop | magnesium chloride | 1.0 (mM) | |
| 5 | 1 | drop | spermine hydrochloride | 0.5 (mM) | |
| 6 | 1 | drop | sodium cacodylate | 40.0 (mM) | |
| 7 | 1 | reservoir | 20 (%) |
