22ZW

norovirus GII24 P domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	73.629, 80.304, 134.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.278 - 2.350
Rwork	0.232
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.325
Data reduction software	HKL-2000 (HLK-2000)
Data scaling software	HKL-2000 (HLK-2000)
Phasing software	PHENIX (PHENIX)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.278	2.411
High resolution limit [Å]	2.350	2.350
Number of reflections	33685	2452
<I/σ(I)>	9.9
Completeness [%]	99.7
Redundancy	10
CC(1/2)	0.640	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M sodium acetate trihydrate (pH 4.0) with 10% (w/v) PEG4,000