22ZA

crystal structures of RipN from Ralstonia solanacearum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 32 2 1
Unit cell lengths	127.355, 127.355, 104.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.278
R-factor	0.26555
Rwork	0.264
R-free	0.29018
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.543
Data reduction software	autoPX
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.278	2.278
Number of reflections	45219	2251
<I/σ(I)>	16.8
Completeness [%]	100.0
Redundancy	11.9
CC(1/2)	1.000	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M sodium cacodylate pH 6.1, 0.2 M magnesium acetate, 20% PEG 8000, 10 mM magnesium chloride