22YX
RufO homolog binding with Nle-Arg-Tyr-Leu-His from Nonomuraea solani
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.976, 78.989, 110.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.290 - 1.590 |
| R-factor | 0.1801 |
| Rwork | 0.179 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.290 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.055 | 0.658 |
| Number of reflections | 52452 | 2472 |
| <I/σ(I)> | 28.6 | |
| Completeness [%] | 100.0 | 95.9 |
| Redundancy | 12.3 | |
| CC(1/2) | 1.000 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M LiSO4, 0.1M Tris-HCl (ph 7.5), 30% PEG 4000 |
