22XH

GII.23/24/25 noroviruses recognize glycans via a conventional glycan-binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97892
Spacegroup name	C 1 2 1
Unit cell lengths	108.950, 53.392, 109.355
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	47.700 - 1.600
Rwork	0.180
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000 (HKL-2000)
Data scaling software	HKL-2000 (HKL-2000)
Phasing software	PHENIX (PHENIX)
Refinement software	PHENIX (PHENIX)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	80852	7979
<I/σ(I)>	2.454
Completeness [%]	98.5
Redundancy	5.5
CC(1/2)	0.811	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.17M ammonium sulfate, 0.085 M sodium cacodylate trihydrate(pH 6.5), 25.5% (w/v) PEG 8,000, and 15% (v/v) glycerol