22WZ

GII.23/24/25 noroviruses recognize glycans via a conventional glycan-binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9789
Spacegroup name	C 1 2 1
Unit cell lengths	108.839, 53.491, 109.425
Unit cell angles	90.00, 102.70, 90.00

Refinement procedure

Resolution	45.310 - 1.500
Rwork	0.184
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	AutoProcess (PDB_EXTRACT)
Data scaling software	HKL-2000 (HKL-2000)
Phasing software	PHENIX (PHENIX)
Refinement software	PHENIX (PHENIX)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.771	1.550
High resolution limit [Å]	1.491	1.500
Number of reflections	99331	9821
<I/σ(I)>	2.955
Completeness [%]	97.9
Redundancy	6.6
CC(1/2)	0.853	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.17M ammonium sulfate, 0.085 M sodium cacodylate trihydrate (pH 6.5), 25.5% (w/v) PEG 8,000, and 15% (v/v) glycerol