22OZ

Structural insights into HpaR-mediated recognition of hrpX and hrpG in Xanthomonas campestris pv. campestris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	106.880, 75.430, 139.490
Unit cell angles	90.00, 112.36, 90.00

Refinement procedure

Resolution	50.130 - 2.511
R-factor	0.2164
Rwork	0.215
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.030
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.130	2.601
High resolution limit [Å]	2.511	2.511
Number of reflections	70271	6930
<I/σ(I)>	16.37
Completeness [%]	99.6
Redundancy	2
CC(1/2)	0.999	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium acetate trihydrate (pH 4.5) and 3.0 M sodium chloride