22OC
Crystal structure of P210 CD lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | H 3 |
| Unit cell lengths | 109.670, 109.670, 51.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.433 - 2.000 |
| Rwork | 0.162 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.576 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.433 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.047 | 0.300 |
| Rmeas | 0.060 | 0.374 |
| Rpim | 0.036 | 0.220 |
| Number of reflections | 15540 | 1151 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.999 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M Tris pH 8.0,1.6 M lithium sulfate |
