22IZ

Crystal structure of MDM2 in complex with St3-R staple peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	46.365, 64.587, 47.619
Unit cell angles	90.00, 117.94, 90.00

Refinement procedure

Resolution	42.070 - 1.520
R-factor	0.1879
Rwork	0.187
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.647
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.070	1.550
High resolution limit [Å]	1.520	1.520
Number of reflections	38207	1884
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	3.4
CC(1/2)	0.995	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	PEG 3350, sodium chloride