22HQ

Crystal structure of GDP-bound FtsZ from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	44.270, 80.840, 84.070
Unit cell angles	101.73, 98.66, 92.52

Refinement procedure

Resolution	79.041 - 2.300
Rwork	0.214
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6umk
RMSD bond length	0.013
RMSD bond angle	1.348
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.410	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	46821	16020
<I/σ(I)>	10.9
Completeness [%]	93.7
Redundancy	3.4
CC(1/2)	0.990	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MIB, pH-9.0, 25% PEG 1500