22HK
Beta-1,2-glucan-binding protein complexed with cyclc beta-1,2-glucononadecaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.488, 83.197, 94.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.278 - 1.270 |
| Rwork | 0.165 |
| R-free | 0.18070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.280 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Number of reflections | 150304 | 7340 |
| <I/σ(I)> | 35.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | |
| CC(1/2) | 1.000 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium sulfate, bis-Tris, cyclic beta-1,2-glucononadecaose |
