22HJ

Beta-1,2-glucan-binding protein soaked with cyclic beta-1,2-glucooctaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	72.699, 82.849, 94.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.992 - 1.600
Rwork	0.167
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.912
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.250	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	75627	3708
<I/σ(I)>	33.9
Completeness [%]	100.0	99.9
Redundancy	12.4
CC(1/2)	1.000	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium sulfate, bis-Tris, cyclic beta-1,2-glucooctaose