22HI

Beta-1,2-glucan-binding protein complexed with cyclic beta-1,2-glucoheptadecaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	72.389, 82.908, 94.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.176 - 1.950
Rwork	0.247
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.477
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	41270	2842
<I/σ(I)>	12.8
Completeness [%]	98.5	97.4
Redundancy	12.4
CC(1/2)	0.997	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium sulfate, bis-Tris, cyclic beta-1,2-glucoheptadecaose