22HH

Beta-1,2-glucan-binding protein complex with linear beta-1,2-glucan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	73.710, 83.261, 94.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.628 - 1.530
Rwork	0.158
R-free	0.17560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.821
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	1.560
High resolution limit [Å]	1.530	1.530
Number of reflections	88131	4318
<I/σ(I)>	18.2
Completeness [%]	100.0	100
Redundancy	6.5
CC(1/2)	0.999	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium sulfate, bis-Tris, linear beta-1,2-glucan