22GX

Crystal structure of the CJ1041C protein from Campylobacter jejuni in the apo form in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97935
Spacegroup name	C 1 2 1
Unit cell lengths	141.547, 56.154, 80.554
Unit cell angles	90.00, 120.06, 90.00

Refinement procedure

Resolution	28.080 - 1.950
R-factor	0.22
Rwork	0.218
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	22gv
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	38518	1557
<I/σ(I)>	14.1	1.3
Completeness [%]	96.8	79.4
Redundancy	3.2	3
CC(1/2)	0.997	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 4000, isopropanol, sodium acetate, EDTA