22GW

Crystal structure of the CJ1041C protein from Campylobacter jejuni in the apo form in space group P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97934
Spacegroup name	P 1 2 1
Unit cell lengths	80.760, 56.396, 121.024
Unit cell angles	90.00, 97.54, 90.00

Refinement procedure

Resolution	29.990 - 2.000
R-factor	0.1886
Rwork	0.187
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	22gv
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	70660	2961
<I/σ(I)>	12.9	1.2
Completeness [%]	96.5	81.2
Redundancy	3.2	2.8
CC(1/2)	0.990	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	Jeffamine, HEPES, EDTA