22GF

Crystal structure of the C-terminal domain of Schizosaccharomyces pombe FKBP nucleoplasmin SpAni2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	H 3 2
Unit cell lengths	113.097, 113.097, 221.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.859 - 2.488
Rwork	0.198
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.871
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	2.590
High resolution limit [Å]	2.490	2.490
Number of reflections	20100	2098
<I/σ(I)>	13.6
Completeness [%]	97.4
Redundancy	5.8
CC(1/2)	0.990	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	200 mM KCl, 50 mM HEPES (pH 7.5), 35% v/v Pentaerythritol propoxylate (5/4 PO/OH)