21XQ

a novel GH8 family endoxylanase BgXyn8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-08
Detector	Nonius Kappa CCD
Wavelength(s)	1.54184
Spacegroup name	P 32 2 1
Unit cell lengths	104.935, 104.935, 76.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.100 - 2.200
R-factor	0.195481698924
Rwork	0.194
R-free	0.22509
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.829
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.000	20.200
High resolution limit [Å]	2.200	2.200
Rmerge	0.037	0.037
Number of reflections	24887	24886
<I/σ(I)>	35.41
Completeness [%]	99.8
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 3350, sodium chloride, HEPES