21NG
structure of IFP35 NID domain dimer form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.642, 36.579, 74.523 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 25.610 - 1.530 |
| R-factor | 0.2134 |
| Rwork | 0.209 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.430 | 1.610 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.121 | 1.503 |
| Rmeas | 0.135 | 1.780 |
| Rpim | 0.059 | 0.930 |
| Total number of observations | 99771 | 8338 |
| Number of reflections | 20421 | 2596 |
| <I/σ(I)> | 7.9 | 1 |
| Completeness [%] | 96.7 | |
| Redundancy | 4.9 | 3.2 |
| CC(1/2) | 0.997 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M HEPES pH7.5, 10% (w/v) polyethylene glycol 3350 |
