21LD

Crystal structure of the ester-hydrolyzing ribozyme (EHR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-06
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.979183
Spacegroup name	P 41 21 2
Unit cell lengths	71.880, 71.880, 82.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.940 - 2.690
R-factor	0.1794
Rwork	0.178
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.936
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.220	2.840
High resolution limit [Å]	2.690	2.690
Rmerge	0.078	1.968
Rmeas		2.047
Rpim	0.024	0.558
Number of reflections	6189	906
<I/σ(I)>	13.9	1.2
Completeness [%]	96.5	100
Redundancy	12.5	13.3
CC(1/2)	0.999	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	0.2 M Ammonium Acetate 0.1 M Sodium cacodylate trihydrate pH 6.2 34% MPD 10mM TCEP Hydrochloride