21KV

Crystal strucrue of HuHF-C2-DAC complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	F 2 3
Unit cell lengths	184.663, 184.663, 184.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.290 - 2.000
R-factor	0.1701
Rwork	0.168
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.151	0.600
Rmeas	0.155	0.617
Rpim	0.035	0.143
Number of reflections	35253	2569
<I/σ(I)>	24.73	7.21
Completeness [%]	100.0
Redundancy	19.28
CC(1/2)	0.997	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Bicine, MgCl2