21EX
Wild tipe p53WT-HLA-A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97881 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.134, 79.485, 60.427 |
| Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
| Resolution | 43.637 - 2.021 |
| R-factor | 0.1611 |
| Rwork | 0.160 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.788 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.640 | 2.093 |
| High resolution limit [Å] | 2.021 | 2.021 |
| Number of reflections | 173333 | 3352 |
| <I/σ(I)> | 21.47 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5 | |
| CC(1/2) | 0.997 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium Chloride, 22% PEG 3000,0.1M HEPES,pH 7.5 |
