21EE

Crystal structure of human PHF20L1(1-80) in complex with H3K36me

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	38.974, 42.410, 47.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.550 - 1.340
R-factor	0.1909
Rwork	0.189
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.560	1.360
High resolution limit [Å]	1.340	1.340
Number of reflections	34026	868
<I/σ(I)>	11.6
Completeness [%]	99.9
Redundancy	1.9
CC(1/2)	0.998	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	PEG 4000 ,0.2 M Malonate pH 6.5