21DC
Crystal structure of poly-gamma-glutamate hydrolase from Bacillus phage PM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 69.424, 69.424, 123.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.090 - 1.200 |
| R-factor | 0.1517 |
| Rwork | 0.151 |
| R-free | 0.15910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.090 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.320 | 3.000 |
| Number of reflections | 216457 | 32866 |
| <I/σ(I)> | 13.5 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 12.9 |
| CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 25% (w/v) Polyethylene glycol 4,000 |
