21CP
Crystal structure of LCDV2 Bcl-2 in complex with the zebrafish BaxA BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2025-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.141, 89.141, 50.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.001 - 2.289 |
| R-factor | 0.1979 |
| Rwork | 0.193 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.728 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.289 | 2.300 |
| Rmerge | 0.116 | 0.301 |
| Number of reflections | 9325 | 437 |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 96.8 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M ammonium citrate dibasic, 20% (w/v) polyethylene glycol 3350 |
