21AZ

Ectodomain of Arabidopsis cell-surface hydrogen peroxide sensor HPCA1/CARD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 2 1
Unit cell lengths	118.852, 41.887, 129.788
Unit cell angles	90.00, 110.83, 90.00

Refinement procedure

Resolution	32.360 - 2.000
R-factor	0.2147
Rwork	0.214
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.360	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.125	0.901
Rmeas	0.137	0.985
Number of reflections	81700	4387
<I/σ(I)>	10.1
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-TRIS-propane ,25% Poly ethylene glycol 1500,0.1 M Sodium chloride ,0.1 M NDSB-221(HAMPTON), 5 mM TCEP.