21AW
Crystal structure of Nicotiana benthamiana importin alpha-Cucumber mosaic virus (strain FNY) suppressor of silencing 2b NLS peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.929, 77.532, 98.575 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 98.570 - 2.000 |
| R-factor | 0.18843 |
| Rwork | 0.187 |
| R-free | 0.22281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.570 | 2.048 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.056 |
| Number of reflections | 64065 | 60824 |
| <I/σ(I)> | 29.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M lithium citrate, 35% glycerol ethoxylate |
