21AI
Crystal structure of Nicotiana benthamiana importin alpha-Beet black scorch virus p7a NLS peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.313, 76.779, 81.704 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 82.310 - 1.990 |
| R-factor | 0.20256 |
| Rwork | 0.201 |
| R-free | 0.22964 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.310 | 2.039 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 65764 | 62438 |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 93.7 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.958 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M lithium citrate, 35% glycerol ethoxylate |
