218D
THE STRUCTURE OF A NEW CRYSTAL FORM OF A DNA DODECAMER CONTAINING T.(O6ME)G BASE PAIRS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 22.120, 38.810, 64.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.250 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.800 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 2387 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 23 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 296.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.0 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | MPD | 15 (%) | |
| 5 | 1 | reservoir | MPD | 30 (%) |
