20ZQ
Crystal structure of rice HPPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.064, 93.436, 113.957 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.126 | |
| Number of reflections | 127948 | 6325 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 98.2 | 98.4 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.988 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | PEG3350 |
