20ZP

Cryogenic crystal structure of p97 N-D1 hexamer in the presence of ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 6 2 2
Unit cell lengths	146.303, 146.303, 85.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.970 - 2.000
R-factor	0.218
Rwork	0.216
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dyi
RMSD bond length	0.007
RMSD bond angle	0.919
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.129	1.239
Number of reflections	36670	2658
<I/σ(I)>	14.9
Completeness [%]	99.9
Redundancy	10.6
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	3.6 M ammonium acetate, 100 mM sodium acetate trihydrate (pH 4.6)