20YG
Crystal structure of Nicotiana benthamiana importin-alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | HEPS BEAMLINE ID02U1A |
| Synchrotron site | HEPS |
| Beamline | ID02U1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-28 |
| Detector | HEPS BPIX40-6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.702, 49.042, 61.820 |
| Unit cell angles | 93.05, 105.62, 93.09 |
Refinement procedure
| Resolution | 59.390 - 1.880 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.23604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.390 | 1.925 |
| High resolution limit [Å] | 1.876 | 1.876 |
| Rmeas | 0.094 | 0.094 |
| Number of reflections | 32273 | 30336 |
| <I/σ(I)> | 25.8 | |
| Completeness [%] | 93.4 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES, pH 7.0, 0.1 M ammonium formate , 20% Sokalan CP 7 |
