208D
HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 22.238, 37.010, 54.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.050 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BDL001 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.500 * |
| Data reduction software | IPMOSFLM |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.050 * |
| Rmerge | 0.036 |
| Number of reflections | 2824 |
| Completeness [%] | 87.0 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 16 * | pH 6.00, VAPOR DIFFUSION, temperature 289.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.5 (mM) | |
| 2 | 1 | drop | 54 (mM) | ||
| 3 | 1 | drop | spermine | 2.4 (mM) | |
| 4 | 1 | drop | MPD | 11 (%) | |
| 5 | 1 | reservoir | MPD | 50 (%) |
