200D
STABLE LOOP IN THE CRYSTAL STRUCTURE OF THE INTERCALATED FOUR-STRANDED CYTOSINE-RICH METAZOAN TELOMERE
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-20 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 59.940, 81.330, 26.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.850 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22300 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 4.000 |
| Data reduction software | MSC |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.045 |
| Completeness [%] | 77.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | pH 6.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | KCL | ||
| 6 | 1 | 1 | K CACODYLATE | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | MPD | 5 (%(v/v)) | ||
| 3 | 1 | spermine | 0.10 (mM) | ||
| 5 | 1 | potassium cacodylate | 40 (mM) | ||
| 6 | 1 | DNA | 2 (mM) | ||
| 7 | 1 | MPD | 35 (%) |
