1ZP7
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-02 |
Detector | RIGAKU |
Wavelength(s) | 0.97 |
Spacegroup name | P 65 |
格子定数 [Å] | 74.340, 74.340, 153.466 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 19.070 - 2.250 |
R因子 | 0.21925 |
Rwork | 0.217 |
R-free | 0.26943 |
Structure solution method | MIR |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 1.320 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Quality characteristics
Overall | |
分解能 [Å] (低) | 19.100 |
分解能 [Å] (高) | 2.250 |
独立反射数 | 22690 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | ||||
1 |