1Z6T
Structure of the apoptotic protease-activating factor 1 bound to ADP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 75.955, 92.883, 94.988 |
| 格子定数 [度] | 62.96, 89.99, 90.05 |
精密化法
| 残基 | 15.000 - 2.210 |
| R因子 | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.270 |
| 分解能 [Å] (高) | 2.200 | 2.210 |
| Rmerge_l_obs | 0.048 | |
| 独立反射数 | 103300 | |
| <I/σ(I)> | 12.7 | 2.5 |
| 完全性 [%] | 89.5 | 48.9 |
| 冗長性 | 1.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7.1 | 276 | HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10 | |
| 1 | 7.1 | 276 | HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10 |
