1Z1Q
Y66L Variant of Enhanced Green Fluorescent Protein with 374-nm Absorbing Chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.926, 62.464, 69.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.670 - 1.500 |
| Rwork | 0.175 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s6z |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | CRYSTAL (VIEW) |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.288 |
| Number of reflections | 33847 | |
| <I/σ(I)> | 10 | 2.6 |
| Completeness [%] | 94.1 | 70.2 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, calcium acetate, sodium chloride, cacodylate, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
