1Z1C

Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute virus of Mice

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	FUJI
Wavelength(s)	0.91
Spacegroup name	C 1 2 1
格子定数 [Å]	448.700, 416.700, 305.300
格子定数 [度]	90.00, 95.80, 90.00

精密化法

残基	20.000 - 3.500
Rwork	0.325
R-free	0.32900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	canine parvovirus model
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.619
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Quality characteristics

	Overall
分解能 [Å] (低)	20.000
分解能 [Å] (高)	3.500
Rmerge_l_obs	0.155
独立反射数	437558
完全性 [%]	63.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5		10mM Tris-HCl, 8 mM CaCl2, PEG8000, virus 10mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP