1YSA
THE GCN4 BASIC REGION LEUCINE ZIPPER BINDS DNA AS A DIMER OF UNINTERRUPTED ALPHA HELICES: CRYSTAL STRUCTURE OF THE PROTEIN-DNA COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 118 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 49.130, 88.540, 59.240 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 8.000 - 2.900 |
| R因子 | 0.23 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.023 |
| 結合角の平均二乗偏差(RMSD) [度] | 3.300 |
| Refinement software | TNT |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 8.000 |
| 分解能 [Å] (高) | 2.900 |
| Rmerge_l_obs | 0.099 |
| Total number of observations | 21237 * |
| 独立反射数 | 5289 |
| 完全性 [%] | 96.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 5.75 | 22 * | well solution contains twice the concentrations of salts and PEG400 * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 3 | PEG 400 | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | MES | ||
| 6 | 1 | 1 | SPERMINE_HCL | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 400 | ||
| 9 | 1 | 3 | WATER |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | pGCNK58 monomer | 0.95 (mM) | |
| 2 | 1 | drop | DNA | 0.57 (mM) | |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | 0.03 (M) | ||
| 5 | 1 | drop | 0.025 (M) | ||
| 6 | 1 | drop | spermine-HCL | 1 (mM) | |
| 7 | 1 | drop | PEG400 | 12 (%(v/v)) |
