1YF2
Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 71.972, 94.222, 103.520 |
| 格子定数 [度] | 90.00, 95.01, 90.00 |
精密化法
| 残基 | 48.520 - 2.400 |
| R因子 | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.290 |
| 分解能 [Å] (高) | 2.210 | 2.210 |
| 独立反射数 | 59867 | |
| 完全性 [%] | 26.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 3000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
