1Y80
Structure of a corrinoid (factor IIIm)-binding protein from Moorella thermoacetica
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.5798 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 55.689, 62.738, 34.543 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 41.630 - 1.700 |
| R因子 | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.21010 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.954 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.02) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 1.660 |
| 分解能 [Å] (高) | 1.600 | 3.450 | 1.600 |
| Rmerge_l_obs | 0.077 | 0.064 | 0.216 |
| 独立反射数 | 13939 | ||
| 完全性 [%] | 89.3 | 38.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 6.7 | 291 | 0.1M Sodium HEPES, 1.6M ammonium sulfate, 30% 1,6-hexanediol, pH 6.7, modified microbatch, temperature 291K |
