1XB9
The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 198.913, 64.549, 97.461 |
Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
Resolution | 91.290 - 1.900 |
R-factor | 0.20794 |
Rwork | 0.204 |
R-free | 0.25504 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k5j |
RMSD bond length | 0.033 |
RMSD bond angle | 2.559 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.038 | 0.221 |
Number of reflections | 89372 | |
<I/σ(I)> | 14.5 | 3.24 |
Completeness [%] | 94.0 | 94 |
Redundancy | 4.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG400, Tris-HCl, Calcium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |