1VPS
POLYOMAVIRUS VP1 PENTAMER COMPLEXED WITH A DISIALYLATED HEXASACCHARIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 221.500, 221.500, 99.800 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 20.000 - 1.900 |
| R因子 | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNCOMPLEXED VP1 PENTAMER (PDB ENTRY 1VPN) |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 25.000 | 2.000 |
| 分解能 [Å] (高) | 1.900 | 1.900 |
| Rmerge_l_obs | 0.058 * | |
| 独立反射数 | 188556 | |
| <I/σ(I)> | 14.5 | 2.1 |
| 完全性 [%] | 85.6 | 56.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8 | DROP: 1.0 M AMMONIUM PHOSPHATE PH 8.0 2.5 & ETHANOL 8-10 MG/ML PROTEIN RESERVOIR: 2.0 M AMMONIUM PHOSPHATE PH 8.0 5 % ETHANOL |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | ammonium phosphate | 1.0 (M) | |
| 2 | 1 | drop | ethanol | 2.5 (%) | |
| 3 | 1 | drop | protein | 8-10 (mg/ml) | |
| 4 | 1 | reservoir | ammonium phosphate | 2.0 (M) | |
| 5 | 1 | reservoir | ethanol | 5 (%) |
