1UJ8
Structures of ORF3 in Two Crystal Forms, a Member of Isc Machinery of E. coli Involved in the Assembly of Iron-Sulfur Clusters
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9740, 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.630, 27.250, 39.680 |
| Unit cell angles | 90.00, 111.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.750 |
| Rwork | 0.194 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 | 0.224 |
| Number of reflections | 7922 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
