1SL7
Crystal structure of calcium-loaded apo-obelin from Obelia longissima
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 58.641, 58.641, 110.378 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 51.990 - 2.200 |
| R因子 | 0.18856 |
| Rwork | 0.186 |
| R-free | 0.23938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jf2 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.280 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.074 | 0.251 |
| 独立反射数 | 18694 | |
| <I/σ(I)> | 20.4 | 4.7 |
| 完全性 [%] | 99.4 | |
| 冗長性 | 5.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 7.5 | 277 | 20% w/v polyethylene glycol 10000, 0.1M HEPES, pH 7.5, modified microbatch, temperature 277K |
