1SL5
Crystal Structure of DC-SIGN carbohydrate recognition domain complexed with LNFP III (Dextra L504).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-22 |
Detector | ADSC |
Wavelength(s) | 1.08940 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.570, 55.370, 29.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.020 - 1.800 |
Rwork | 0.179 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 12903 | |
Completeness [%] | 93.9 | 88.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 4000, 0.2M MgCl2, 0.1M Tris pH 8.5. Protein solution: 10 mg/ml protein, 5mM CaCl2, 10mM oligosaccharide., VAPOR DIFFUSION, HANGING DROP, temperature 298K |